Wannisa Khamaikawin, Ph.D.
Instructor, Faculty of Medicine
King Mongkut’s Institute of Technology Ladkrabang
B.S. (Medical Technology), 2009
Chiang Mai University
Ph.D. (Biomedical Science), 2015
Chiang Mai University
Ph.D. Student Internship
The Scripts Research Institute (TSRI), La Jolla, California, USA
Chiang Mai University, Thailand
University of California, Los Angeles (UCLA), California, USA
My research of interest is developing anti-HIV gene therapies applying with stem cell therapy to cure HIV. Nowadays AIDS causing by HIV infection still cannot be completely cured by current anti-retroviral therapy. Only two cases in the would have been proved that they are cured of HIV by receiving stem cells transplantation from donor who has HIV receptor mutation nationally. However, finding donors who have matched and HIV resistant cells is extraordinary rare. Hence, I am interested in finding alternative ways to modify human stem cells to resist to HIV infection by using gene therapy approaches. Lentiviral vectors, adenoviral vector, and CRISRPR/cas9 gene editing are current technology that scientists around the world and I have been utilizing to modify or edit human chromosome. Delivery of anti-HIV genes or modify stem cell gene is not easy. New approach using chemotherapy to select only cells receiving modified gene is one of my expertise. Working with me, you will learn how to do gene cloning in bacteria, human cells and stem cells handling, gene editing and characterization in human cells, lentiviral and adenoviral vector productions, chemotherapy gene selection, and CRISPS/Cas9 gene editing, and flow cytometry.
1. Ladinsky MS, Khamaikawin W, Jung Y, Lin S, Lam J, An DS, Bjorkman PJ, Kieffer C. Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice. Elife. 2019 Oct;8.
2. Khamaikawin W, Shimizu S, Kamata M, Cortado R, Jung Y, Lam J, et al. Modeling Anti-HIV-1 HSPC-Based Gene Therapy in Humanized Mice Previously Infected with HIV-1. Mol Ther Methods Clin Dev. 2018 Jun 15;9:23-32.
3. Kitidee K, Khamaikawin W, Thongkum W, Tawon Y, Cressey TR, Jevprasesphant R, et al. Expedient screening for HIV-1 protease inhibitors using a simplified immunochromatographic assay. J Chromatogr B Analyt Technol Biomed Life Sci. 2016 May 15;1021:153-8.
4. Khamaikawin W, Saoin S, Nangola S, Chupradit K, Sakkhachornphop S, Hadpech S, et al. Combined Antiviral Therapy Using Designed Molecular Scaffolds Targeting Two Distinct Viral Functions, HIV-1 Genome Integration and Capsid Assembly. Mol Ther Nucleic Acids. 2015 Aug 25;4:e249.4.
5. Thammasit P, Sangboonruang S, Suwanpairoj S, Khamaikawin W, Intasai N, Kasinrerk W, et al. Intracellular Acidosis Promotes Mitochondrial Apoptosis Pathway: Role of EMMPRIN Down-regulation via Specific Single-chain Fv Intrabody. J Cancer. 2015;6(3):276-86.